Purification of muscle glycogen particles by glycerol-gradient centrifugation
نویسندگان
چکیده
منابع مشابه
The hydrolysis of glycogen by a glycerol extract of muscle.
BARBOUR [1929] found that when glycogen is hydrolysed by a glycerol extract of skeletal muscle or of liver, the reducing activity appearing, expressed as glucose, is only about 30% of the glycogen disappearing. Case [1931] and Gray [1935] were able to confirm this observation, but Carruthers [1935] did not obtain consistent results. From the products of hydrolysis Barbour isolated, by a relativ...
متن کاملextremal region detection guided by maxima of gradient magnitude
a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
Purification of bacterial endotoxins by zonal centrifugation.
In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen....
متن کاملSeparation of Thyroidal Iodoproteins and Purification of Thyroglobulin by Gel Filtration and Density Gradient Centrifugation.
Thyroglobulin, the major protein component of normal thyroid glands, contains more than 80% of the thyroidal iodine and has been considered the only source of thyroid hormones (1). Aqueous thyroid extracts contain, besides thyroglobulin, other proteins in variable amounts. Essentially, four methods have been used for the characterization of the various components in such extracts and for the pu...
متن کاملPurification of large quantities of influenza virus by density gradient centrifugation.
New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80952-6